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Role of Wnt and Notch signaling in regulating hair cell regeneration in the cochlea
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《医学前沿(英文)》 2016年 第10卷 第3期 页码 237-249 doi: 10.1007/s11684-016-0464-9
Sensory hair cells in the inner ear are responsible for sound recognition. Damage to hair cells in adult mammals causes permanent hearing impairment because these cells cannot regenerate. By contrast, newborn mammals possess limited regenerative capacity because of the active participation of various signaling pathways, including Wnt and Notch signaling. The Wnt and Notch pathways are highly sophisticated and conserved signaling pathways that control multiple cellular events necessary for the formation of sensory hair cells. Both signaling pathways allow resident supporting cells to regenerate hair cells in the neonatal cochlea. In this regard, Wnt and Notch signaling has gained increased research attention in hair cell regeneration. This review presents the current understanding of the Wnt and Notch signaling pathways in the auditory portion of the inner ear and discusses the possibilities of controlling these pathways with the hair cell fate determiner Atoh1 to regulate hair cell regeneration in the mammalian cochlea.
关键词: inner ear cochlea hair cell regeneration Wnt Notch signaling pathways
Regeneration of hair cells in the mammalian vestibular system
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《医学前沿(英文)》 2016年 第10卷 第2期 页码 143-151 doi: 10.1007/s11684-016-0451-1
Hair cells regenerate throughout the lifetime of non-mammalian vertebrates, allowing these animals to recover from hearing and balance deficits. Such regeneration does not occur efficiently in humans and other mammals. Thus, balance deficits become permanent and is a common sensory disorder all over the world. Since Forge and Warchol discovered the limited spontaneous regeneration of vestibular hair cells after gentamicin-induced damage in mature mammals, significant efforts have been exerted to trace the origin of the limited vestibular regeneration in mammals after hair cell loss. Moreover, recently many strategies have been developed to promote the hair cell regeneration and subsequent functional recovery of the vestibular system, including manipulating the Wnt, Notch and Atoh1. This article provides an overview of the recent advances in hair cell regeneration in mammalian vestibular epithelia. Furthermore, this review highlights the current limitations of hair cell regeneration and provides the possible solutions to regenerate functional hair cells and to partially restore vestibular function.
Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice
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《医学前沿(英文)》 2016年 第10卷 第4期 页码 481-489 doi: 10.1007/s11684-016-0494-3
The tumor suppressor gene liver kinase B1 (LKB1), also called STK11, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice (LKB1Pax2 CKO mice) were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity (PCP) of hair cells. LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.
关键词: LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
《农业科学与工程前沿(英文)》 2016年 第3卷 第1期 页码 72-80 doi: 10.15302/J-FASE-2016089
Behavioral methods for the functional assessment of hair cells in zebrafish
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《医学前沿(英文)》 2017年 第11卷 第2期 页码 178-190 doi: 10.1007/s11684-017-0507-x
Zebrafish is an emerging animal model for studies on auditory system. This model presents high comparability with humans, good accessibility to the hearing organ, and high throughput capacity. To better utilize this animal model, methodologies need to be used to quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows: the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.
关键词: zebrafish (Danio rerio) behavior auditory startle response vestibular-ocular reflex rheotaxis
LI Shunjiang, YANG Linsheng, WANG Wuyi, LI Yonghua, LI Hairong, XIRAO Ruodeng
《医学前沿(英文)》 2007年 第1卷 第2期 页码 223-225 doi: 10.1007/s11684-007-0043-1
关键词: different relationship non-disease selenium KBD distribution
《医学前沿(英文)》 2022年 第16卷 第4期 页码 637-650 doi: 10.1007/s11684-021-0864-3
关键词: apigenin aminoglycosides ototoxicity oxidative stress Nrf2 signaling pathway
Identification and selection of resistance to
Lizhen ZHU, Jianying LI, Zhongping XU, Hakim MANGHWAR, Sijia LIANG, Suli LI, Muna ALARIQI, Shuangxia JIN, Xianlong ZHANG
《农业科学与工程前沿(英文)》 2018年 第5卷 第2期 页码 236-252 doi: 10.15302/J-FASE-2018223
关键词: Bemisia tabaci Gossypium genotypes gossypol leaf hair density leaf hair length
Alternative splicing of inner-ear-expressed genes
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《医学前沿(英文)》 2016年 第10卷 第3期 页码 250-257 doi: 10.1007/s11684-016-0454-y
Alternative splicing plays a fundamental role in the development and physiological function of the inner ear. Inner-ear-specific gene splicing is necessary to establish the identity and maintain the function of the inner ear. For example, exon 68 of Cadherin 23 (Cdh23) gene is subject to inner-ear-specific alternative splicing, and as a result, Cdh23(+68) is only expressed in inner ear hair cells. Alternative splicing along the tonotopic axis of the cochlea contributes to frequency tuning, particularly in lower vertebrates, such as chickens and turtles. Differential splicing of Kcnma1, which encodes for the α subunit of the Ca2+-activated K+ channel (BK channel), has been suggested to affect the channel gating properties and is important for frequency tuning. Consequently, deficits in alternative splicing have been shown to cause hearing loss, as we can observe in Bronx Waltzer (bv) mice and Sfswap mutant mice. Despite the advances in this field, the regulation of alternative splicing in the inner ear remains elusive. Further investigation is also needed to clarify the mechanism of hearing loss caused by alternative splicing deficits.
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
《医学前沿(英文)》 2019年 第13卷 第6期 页码 690-704 doi: 10.1007/s11684-018-0638-8
关键词: TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 90-98 doi: 10.1007/s11705-015-1550-2
关键词: microfluidic chip co-culture cell-cell interactions review
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
《医学前沿(英文)》 页码 939-956 doi: 10.1007/s11684-023-0987-9
关键词: mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration
Deubiquitinases as pivotal regulators of T cell functions
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《医学前沿(英文)》 2018年 第12卷 第4期 页码 451-462 doi: 10.1007/s11684-018-0651-y
T cells efficiently respond to foreign antigens to mediate immune responses against infections but are tolerant to self-tissues. Defect in T cell activation is associated with severe immune deficiencies, whereas aberrant T cell activation contributes to the pathogenesis of diverse autoimmune and inflammatory diseases. An emerging mechanism that regulates T cell activation and tolerance is ubiquitination, a reversible process of protein modification that is counter-regulated by ubiquitinating enzymes and deubiquitinases (DUBs). DUBs are isopeptidases that cleave polyubiquitin chains and remove ubiquitin from target proteins, thereby controlling the magnitude and duration of ubiquitin signaling. It is now well recognized that DUBs are crucial regulators of T cell responses and serve as potential therapeutic targets for manipulating immune responses in the treatment of immunological disorders and cancer. This review will discuss the recent progresses regarding the functions of DUBs in T cells.
关键词: deubiquitinase ubiquitination T cell activation T cell differentiation T cell tolerance
Cell surface protein engineering for high-performance whole-cell catalysts
Hajime Nakatani,Katsutoshi Hori
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 46-57 doi: 10.1007/s11705-017-1609-3
关键词: cell surface engineering surface display whole-cell catalysts bioprocess
Stem cell niches and endogenous electric fields in tissue repair
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 40-44 doi: 10.1007/s11684-011-0108-z
Adult stem cells are responsible for homeostasis and repair of many tissues. Endogenous adult stem cells reside in certain regions of organs, known as the stem cell niche, which is recognized to have an important role in regulating tissue maintenance and repair. In wound healing and tissue repair, stem cells are mobilized and recruited to the site of wound, and participate in the repair process. Many regulatory factors are involved in the stem cell-based repair process, including stem cell niches and endogenous wound electric fields, which are present at wound tissues and proved to be important in guiding wound healing. Here we briefly review the role of stem cell niches and endogenous electric fields in tissue repair, and hypothesize that endogenous electric fields become part of stem cell niche in the wound site.
标题 作者 时间 类型 操作
MicroRNA-148b promotes proliferation of hair follicle cells by targeting
Wanbao YANG,Qinqun LI,Bo SU,Mei YU
期刊论文
Relationship between selenium concentration in child hair and the distribution of Kashin-Beck Disease
LI Shunjiang, YANG Linsheng, WANG Wuyi, LI Yonghua, LI Hairong, XIRAO Ruodeng
期刊论文
Apigenin alleviates neomycin-induced oxidative damage via the Nrf2 signaling pathway in cochlear hair
期刊论文
Identification and selection of resistance to
Lizhen ZHU, Jianying LI, Zhongping XU, Hakim MANGHWAR, Sijia LIANG, Suli LI, Muna ALARIQI, Shuangxia JIN, Xianlong ZHANG
期刊论文
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
期刊论文
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
期刊论文
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
期刊论文
Cell surface protein engineering for high-performance whole-cell catalysts
Hajime Nakatani,Katsutoshi Hori
期刊论文